Journal:

Article Title: Expression of Scavenger Receptor Class B, Type I, by Astrocytes and Vascular Smooth Muscle Cells in Normal Adult Mouse and Human Brain and in Alzheimer's Disease Brain

doi:

Figure Lengend Snippet: List of Primary and Secondary Antibodies and Control Antibodies Used in First (I) and Second (II) Staining Cycle for Double Labeling

Article Snippet: Polyclonal rabbit anti-SR-BI/BII (RED-I; 1:100) (Novus Biologicals) , 2k.

Techniques: Control, Staining

Journal: Biology of reproduction

Article Title: Differential steroidogenic gene expression in the fetal adrenal gland versus the testis and rapid and dynamic response of the fetal testis to di(n-butyl) phthalate.

doi: 10.1095/biolreprod.105.042382

Figure Lengend Snippet: FIG. 8. Effects of DBP on steroidogenesis, gene expression, and protein expression in the fetal adrenal. A) Total adrenal corticosterone was mea- sured by RIA. Results are expressed as average litter mean corticosterone per adrenal 6 SEM; n 5 4 litters, 2 fetuses per litter. B) Real-time RT-PCR analysis of genes previously shown to be altered in the testis following DBP exposure. Results are expressed as mean normalized expression rel- ative to female controls. Error bars show SEM; n 5 4–5 fetuses per group, with each fetus taken from a separate litter. C) Densitometric quantitation of protein expression for STAR, SCARB1, and CYP11A1. Total protein ly- sates were prepared, and 30 mg of each sample was electorphoresed on 10% SDS-PAGE gels. Error bars show SEM; n 5 4 fetuses per group, with each fetus taken from a separate litter. *P , 0.05 compared to gender- matched control by Student t-test.

Article Snippet: The following antibodies were used to probe the membrane: rabbit anti-mouse SCARB1 (Novus Biologicals, Inc., Littleton, CO), rabbit anti-rat cytochrome CYP11A1 (US Biological, Swampscott, MA), rabbit anti-rat STAR (Affinity Bioreagents, Inc., Golden, CO), and rabbit anti-porcine CYP17A1 (a kind gift from Dr. D.B.

Techniques: Gene Expression, Expressing, Quantitative RT-PCR, Quantitation Assay, SDS Page, Control